Comparison the therapeutic effects of bone marrow CD144⁺ endothelial cells and CD146⁺ mesenchymal stem cells in POF rats

Introduction: Premature ovarian insufficiency (POI) is a challenging issue in terms of reproduction biology. In this study, therapeutic properties bone marrow CD146+ mesenchymal stem cells (MSCs) and CD144+ endothelial (ECs) were separately investigated rats with POI. Methods: POI classified into control POI, + MSCs, ECs groups. Enriched MSCs directly injected tissue (15 × 104 cells/10 μL) relevant After 4 weeks, follicle-stimulating hormone (FSH), luteinizing (LH), estradiol (E2) levels measured blood samples. Ovarian tissues collected subjected to Hematoxylin-Eosin Masson’s trichrome staining. The expression angp-2, vegfr-2, smad-2, -4, -6, tgf-β1 was studied using qRT-PCR analysis. Histopathological examination indicated an increased pattern atretic follicles the group related (P<0.0001). Results: Data that injection reduced number normal (P<0.01). Along these changes, content blue-colored collagen fibers diminished after cell transplantation. Besides, transplantation had potential reduce FSH, LH (P<0.05). contrast, E2 groups compared rats, indicating restoration follicular function. (smad-2, -4) (smad-6) altered activity genes belonging TGF-β signaling pathway. Unlike aberrant angiogenesis significantly down-regulated Conclusion: can lead function via modulating different mechanisms associated fibrosis